cell culture crc cell lines dld1  (DSMZ)

Journal: Signal transduction and targeted therapy

Article Title: Fusobacterium nucleatum enhances the efficacy of PD-L1 blockade in colorectal cancer.

doi: 10.1038/s41392-021-00795-x

Figure Lengend Snippet: Fig. 4 F. nucleatum induces the expression of PD-1 and PD-L1. a Flow cytometry was used to detect the expression of PD-1 in tumor tissues from mice treated with F. nucleatum and/or an anti-PD-L1 mAb. One-way ANOVA and Bonferroni’s multiple comparison test. b The protein levels of PD-L1 were detected by IHC, and F. nucleatum was detected by FISH in tumor tissue samples from mice. Brown staining in IHC and red staining in FISH (white arrows) indicate positive staining. c, d DLD1 and Caco-2 cells were treated with Fn (1:1000) for different time course, and the mRNA and protein levels of PD-L1 were detected by RT-PCR and Western blotting, respectively. Student’s t-test. e DLD1 cells were treated with different dilutions of different Fn isolates obtained from CRC patients. f DLD1 cells were treated with Fn (1:1000) for different time course. g DLD1 cells were treated with Fn (1:1000) and/or 5 μM BAY 11–7082 for 24 h. The expression of the indicated proteins was detected by Western blotting. Actin was used as a loading control. Bars represent s.d. of at least three experiments. *P < 0.05; **P < 0.01. Fn, F. nucleatum. NS, no significant difference

Article Snippet: Cell culture Human CRC cell lines DLD1 and Caco-2 and mouse colon cancer cell line CT26.WT were purchased from the American Type Culture Collection.

Techniques: Expressing, Flow Cytometry, Comparison, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Signal transduction and targeted therapy

Article Title: Fusobacterium nucleatum enhances the efficacy of PD-L1 blockade in colorectal cancer.

doi: 10.1038/s41392-021-00795-x

Figure Lengend Snippet: Fig. 5 F. nucleatum enhanced the therapeutic effect of PD-L1 blockade by activating STING signaling. a–d The indicated proteins were detected by Western blotting. Actin was used as a loading control. a DLD1 cells were treated with Fn (1:1000) for different time course. b DLD1 cells were pre-treated with H151 for 2 h, and then treated with Fn (1:1000) for 12 h. c CT26.WT cells were treated with Fn (1:1000) for different time course. d CT26.WT cells were pre-treated with C176 for 2 h, and then treated with Fn (1:1000) for different time course. e–h CT26.WT cells were subcutaneously injected into BALB/c mice (n = 7 for each group). Tumor-bearing mice were pre-treated with C176 or vehicle, then intratumorally injected with F. nucleatum or PBS and intraperitoneally injected treated with an anti-PD-L1 mAb or an isotype control mAb every three days until the end of the experiment. Tumor volumes were measured. e An image of tumors collected at the end of the experiment is shown. f, g Tumor volumes and relative tumor volumes at various time points are shown. One-way ANOVA and Bonferroni’s multiple comparison test. h Flow cytometry was used to detect the proportion of IFN-γ+ cells in CD8+ TILs from mice. One-way ANOVA and Bonferroni’s multiple comparison test. *P < 0.05; **P < 0.01. Fn, F. nucleatum

Article Snippet: Cell culture Human CRC cell lines DLD1 and Caco-2 and mouse colon cancer cell line CT26.WT were purchased from the American Type Culture Collection.

Techniques: Western Blot, Control, Injection, Comparison, Flow Cytometry